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resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat  (Bethyl)


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    Bethyl resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat
    Figure 5. <t>Sam68</t> Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.
    Resource Source Identifier Antibodies Rabbit Polyclonal Anti Sam68 Bethyl Laboratories Cat, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+antibodies+rabbit+polyclonal+anti+sam68+bethyl+laboratories+cat/SAM68+Antibody/pm32492419-227-2-9
    Average 93 stars, based on 14 article reviews
    resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "A Dynamic Splicing Program Ensures Proper Synaptic Connections in the Developing Cerebellum."

    Article Title: A Dynamic Splicing Program Ensures Proper Synaptic Connections in the Developing Cerebellum.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.107703

    Figure 5. Sam68 Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.
    Figure Legend Snippet: Figure 5. Sam68 Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Control, Two Tailed Test, Western Blot, Expressing

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    Article Title: A Dynamic Splicing Program Ensures Proper Synaptic Connections in the Developing Cerebellum.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Sam68 Bethyl Laboratories Cat#A302-110A; RRID:AB_1604289 Donkey whole antibody anti Rabbit IgG HRP-linked GE Healthcare Cat# NA934; RRID: AB_772206 Sheepwhole antibody anti mouse IgGHRPlinked GE Healthcare Cat# NA931; RRID:AB_772210 Alexa Fluor-488 goat anti mouse Thermo Fisher Scientific Cat# A11001; RRID:AB_2534069 Alexa Fluor-594 goat anti-rabbit Thermo Fisher Scientific Cat# A11037; RRID:AB_2534095 Mouse monoclonal anti 5-bromo-20deoxyuridine (BrdU) Millipore Cat# 05-633, RRID:AB_11212826 Mouse monoclonal anti CALB1 Sigma-Aldrich Cat# C9848, RRID:AB_476894 Mouse monoclonal anti-U2AF65 Sigma-Aldrich Cat#U4758, RRID:AB_262122 Rabbit polyclonal anti VGLUT1 Synaptic System Cat# 135 303, RRID:AB_887875 Mouse monoclonal anti-ACTIN Santa Cruz Cat# sc-47778, RRID: AB_626632 Chemicals, Peptides, and Recombinant Proteins Dnase I from bovine pancreas Sigma Aldrich Cat# DN25; CAS 9003-98-9 Trypsin from bovine pancreas Sigma Aldrich Cat#T8003 Poly-L-lysine Sigma Aldrich Cat# P9155 Trypsin inhibitor from bovine pancreas Sigma Aldrich Cat#T9003 5-Bromo-20-deoxyuridine Sigma Aldrich Cat#B5002 B27tm Supplement Thermo Fisher Scientific,GIBCO Cat#17504-044 (+)-Bicuculline Tocris Cat#0130 Tetrodotoxin Tocris Cat#1078 RNAprotect Tissue Reagent QIAGEN Cat# 76104 Tissue-Tek O.C.T. .. Compound VWR Cat# 25608-930 RNase away Sigma Aldrich Cat# 83931 Proteinase K Sigma Aldrich Cat# 03115879001 Protease inhibitor cocktail Sigma Aldrich Cat# P8340 DNase RNase-free Turbo Thermo Fisher Scientific, Ambion Cat# AM2239 RNasin Ribonuclease Inhibitor Promega Cat# N251B Critical Commercial Assays miRNEasy minikit QIAGEN Cat# 217004 M-MLV reverse transcriptase Promega Cat# M170B SuperScriptTM-IV VILOTM master Mix with EZ DNaseTM Thermo Fisher Scientific, Invitrogen Cat# 11766050 GoTaq Promega Cat# M3005 T7 RNA Polymerase Thermo Fisher Scientific, Ambion Cat# AM2085 RNasin Ribonuclease Inhibitor Promega Cat# N251B LightCycler 480 SYBR Green I Master Roche Cat# 04 887 352 001 Clarity Western ECL Blotting Substrate Biorad Cat#1705060 RNase I Thermo Fisher Scientific, Invitrogen Cat#AM2295 RNA Labeling Mix Roche Cat# 11685597910 Streptavidin-Sepharose high performance Sigma Aldrich Cat# GE17-5113-01 Dynabeads Protein G Thermo Fisher Scientific, Novex Cat#10004D 4’,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific, Cat#D1306 (Continued on next page) Cell Reports 31, 107703, June 2, 2020 e1



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    resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat  (Bethyl)
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    Bethyl resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat
    Figure 5. <t>Sam68</t> Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.
    Resource Source Identifier Antibodies Rabbit Polyclonal Anti Sam68 Bethyl Laboratories Cat, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+antibodies+rabbit+polyclonal+anti+sam68+bethyl+laboratories+cat/SAM68+Antibody/pm32492419-227-2-9
    Average 93 stars, based on 1 article reviews
    resource source identifier antibodies rabbit polyclonal anti sam68 bethyl laboratories cat - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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    Figure 5. Sam68 Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.

    Journal: Cell reports

    Article Title: A Dynamic Splicing Program Ensures Proper Synaptic Connections in the Developing Cerebellum.

    doi: 10.1016/j.celrep.2020.107703

    Figure Lengend Snippet: Figure 5. Sam68 Preferentially Represses Exons Characterized by a Weak Branchpoint 5368289352488500(A and B) Branchpoint score (A) and GC content (B) for up- and downregulated exons in Sam68KO cerebella with respect to non-regulated exons (Wilcoxon rank-sum test). (C) Distribution of the DPSI of exons differentially regulated between Sam68KO and WT cerebella. Whiskers indicate 1.5 interquartile range. Welch’s t test; ****p < 0.0001. (D and E) Left panels: scheme of the AS region of the Arhgef9 (D) and Arhgap12 (E) gene, with regulated (red) and constitutive exons (green) and qPCR primers (arrows) and the splicing events in WT and Sam68KO cerebella. Shown is RT-PCR analysis for Sam68-regulated events in Arhgef9 (D) and Arhgap12 (E) with PSI values from densitometric analysis (mean ± SEM, n = 6, paired t test). Right panels: bar graphs showing qPCR analyses of Sam68 CLIPs for Arhgef9 (D) and Arhgap12 (E) pre-mRNAs in P10 mouse cerebella. Immunoglobulin G (IgG) was used as a control (mean ± SEM, n = 5, two tailed paired t test, *p < 0.05). (F and G) qPCR analyses of U2AF65 CLIPs for Arhgef9 (F) and Arhgap12 (G) pre-mRNAs in WT and Sam68KO P10 cerebella (mean ± SEM, n = 5, multiple Student’s t test, *p < 0.05). (H) Western blot analysis of U2AF65 and Sam68 expression in WT and Sam68KO P10 mouse cerebella. See also Figure S5.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Sam68 Bethyl Laboratories Cat#A302-110A; RRID:AB_1604289 Donkey whole antibody anti Rabbit IgG HRP-linked GE Healthcare Cat# NA934; RRID: AB_772206 Sheepwhole antibody anti mouse IgGHRPlinked GE Healthcare Cat# NA931; RRID:AB_772210 Alexa Fluor-488 goat anti mouse Thermo Fisher Scientific Cat# A11001; RRID:AB_2534069 Alexa Fluor-594 goat anti-rabbit Thermo Fisher Scientific Cat# A11037; RRID:AB_2534095 Mouse monoclonal anti 5-bromo-20deoxyuridine (BrdU) Millipore Cat# 05-633, RRID:AB_11212826 Mouse monoclonal anti CALB1 Sigma-Aldrich Cat# C9848, RRID:AB_476894 Mouse monoclonal anti-U2AF65 Sigma-Aldrich Cat#U4758, RRID:AB_262122 Rabbit polyclonal anti VGLUT1 Synaptic System Cat# 135 303, RRID:AB_887875 Mouse monoclonal anti-ACTIN Santa Cruz Cat# sc-47778, RRID: AB_626632 Chemicals, Peptides, and Recombinant Proteins Dnase I from bovine pancreas Sigma Aldrich Cat# DN25; CAS 9003-98-9 Trypsin from bovine pancreas Sigma Aldrich Cat#T8003 Poly-L-lysine Sigma Aldrich Cat# P9155 Trypsin inhibitor from bovine pancreas Sigma Aldrich Cat#T9003 5-Bromo-20-deoxyuridine Sigma Aldrich Cat#B5002 B27tm Supplement Thermo Fisher Scientific,GIBCO Cat#17504-044 (+)-Bicuculline Tocris Cat#0130 Tetrodotoxin Tocris Cat#1078 RNAprotect Tissue Reagent QIAGEN Cat# 76104 Tissue-Tek O.C.T.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Two Tailed Test, Western Blot, Expressing